MOPS
Chemical compound
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
01Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.
Sources and credits
This article is adapted from the Wikipedia article “MOPS”, written by its contributors and licensed under CC BY-SA 4.0. Fathomly has changed the layout, removed citation markers, navigation and maintenance notices, and adjusted punctuation. This adapted version is shared under the same license. For references, see the original article.
Images, from Wikimedia Commons:
- Mops is.svg by Codc, Public domain
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