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Methoxy arachidonyl fluorophosphonate

Chemical compound

Wikipedia contributorsAdapted for Fathomly · View authors
1 min read
Source updated December 26, 2025
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Methoxy arachidonyl fluorophosphonate, commonly referred as MAFP, is an irreversible active site-directed enzyme inhibitor that inhibits nearly all serine hydrolases and serine proteases. It inhibits phospholipase A2 and fatty acid amide hydrolase with special potency, displaying IC50 values in the low-nanomolar range. In addition, it binds to the CB1 receptor in rat brain membrane preparations (IC50 = 20 nM), but does not appear to agonize or antagonize the receptor, though some related derivatives do show cannabinoid-like properties.

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This article is adapted from the Wikipedia article “Methoxy arachidonyl fluorophosphonate”, written by its contributors and licensed under CC BY-SA 4.0. Fathomly has changed the layout, removed citation markers, navigation and maintenance notices, and adjusted punctuation. This adapted version is shared under the same license. For references, see the original article.

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  • Mafp.png by Roadnottaken at en.wikipedia, Public domain

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Diisopropyl fluorophosphate

Diisopropyl fluorophosphate or Isofluorophate is an oily, colorless liquid with the chemical formula C6H14FO3P. It was once used to treat glaucoma and is currently used as an organophosphorus insecticide. It is stable, but undergoes hydrolysis when subjected to moisture.

IDFP

IDFP is an organophosphorus compound related to the nerve agent sarin. Like sarin, IDFP is an irreversible inhibitor for a number of different enzymes that normally serve to break down neurotransmitters, however the long alkyl chain of IDFP makes it dramatically weaker as an inhibitor of acetylcholinesterase, with an IC50 of only 6300 nM, while it is a potent inhibitor of two enzymes monoacylglycerol lipase (MAGL), the primary enzyme responsible for degrading the endocannabinoid 2-arachidonoylglycerol (2-AG), and fatty acid amide hydrolase (FAAH), the primary enzyme that degrades the other main endocannabinoid anandamide.

Activity-based proteomics

Activity-based proteomics, or activity-based protein profiling is a chemoproteomic strategy that employs modular probes to directly assess the functional state of enzymes within complex proteomes. Unlike expression-based proteomics, which measures protein abundance, ABPP provides information about catalytic activity, thereby enabling researchers to distinguish between active and inactive forms of enzymes.