Flo

• Flo (name), a list of people with the name Flo
• Flo, nickname of LB1, a Homo floresiensis fossil

• Flo, Norway, a village near Stryn, Vestland
• Flo, Sweden, a historic parish near Grästorp, Västergötland
• Flo, Texas, an unincorporated community in Leon County, Texas, United States

Film and television

• Flo (TV series), an American TV sitcom
• FLO TV, an American mobile television service

Fictional characters

• Flo (Progressive Insurance), in commercials for the American insurance company Progressive
• Florence Jean Castleberry, or Flo, first introduced in the 1974 film Alice Doesn't Live Here Anymore
• Flo, title character of the comic strip Flo & Friends
• Flo, a humbug damselfish from the Finding Nemo franchise
• Flo (Cars), a character from the Cars franchise
• Flo, a character from the video game Diner Dash and its franchise.
• The Flo, companions of Tina and Milo, the mascots of the 2026 Winter Olympics and 2026 Winter Paralympics

Music

• Flo (group), a British girl group
• "Flo", a 2000 song by Smash Mouth from the album Fush Yu Mang
• "Flo", a 1989 song by Pierre Bachelet from the album Quelque part... c'est toujours ailleurs

• Flo (app), a menstruation, ovulation and pregnancy monitoring mobile application
• FLO charging network a Quebec-based electric vehicle charging network
• Flo (chimpanzee), a member of the Kasakela chimpanzee community
• FLO (retailer), a chain of footwear stores based in Turkey

• Florence Regional Airport (IATA code FLO), South Carolina, U.S.
• Florence station (South Carolina), U.S.; station code FLO
• Fairtrade International, or Fairtrade Labelling Organizations International e.V. (FLO)
• Flowers Foods (New York Stock Exchange symbol FLO)
• Family Liaison Officer, a role in the British police