Electroelution
Electroelution is a method used to extract a nucleic acid or a protein sample from an electrophoresis gel by applying a negative current in the plane of the smallest dimension of the gel, drawing the macromolecule to the surface for extraction and subsequent analysis. For example, electroblotting is based upon the same principle.
01DNA extraction
Using this method, DNA fragments can be recovered from a particular region of agarose or polyacrylamide gels. The gel piece containing the fragment is excised (cut out from the whole gel) and placed in a dialysis bag with buffer. Electrophoresis causes the DNA to migrate out of the gel into the dialysis bag buffer. The DNA fragments are recovered from this buffer and purified, using phenol-chloroform extraction followed by ethanol precipitation. This method is simple, rapid and yields high recovery (75%) of DNA fragments from gel pieces.
02Protein extraction
Preparative native PAGE yields more than 95% recovery of metalloproteins. Proteins are continuously eluted from a gel column and separated in different fractions according to their isoelectric points.
Sources and credits
This article is adapted from the Wikipedia article “Electroelution”, written by its contributors and licensed under CC BY-SA 4.0. Fathomly has changed the layout, removed citation markers, navigation and maintenance notices, and adjusted punctuation. This adapted version is shared under the same license. For references, see the original article.
Images, from Wikimedia Commons:
- Electrphoresis Tools.TIF by Indeblues, CC BY-SA 3.0
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